ICS65.020.20 CCSB16 2301 黑龙江省哈尔滨市地方标准 DB2301/T200—2024 大豆疫霉种群动态监测技术规程 2024-12-30发布 2025-02-05实施 哈尔滨市市场监督管理局  发布 DB2301/T200—2024 I目次 前言........................................................................................................................................................................II 1范围...................................................................................................................................................................1 2规范性引用文件..............................................................................................................................................1 3术语和定义......................................................................................................................................................1 4田间样本采集..................................................................................................................................................1 4.1取样地点...................................................................................................................................................1 4.2取样时期...................................................................................................................................................1 4.3取样方法...................................................................................................................................................1 4.4样本记录...................................................................................................................................................1 5室内检测..........................................................................................................................................................2 5.1大豆疫霉分离及鉴定...............................................................................................................................2 5.2菌株的纯化...............................................................................................................................................2 5.3菌株的保存...............................................................................................................................................2 6生理小种鉴定..................................................................................................................................................2 6.1大豆植株的培养.......................................................................................................................................2 6.2接种...........................................................................................................................................................2 6.3病情调查...................................................................................................................................................2 6.4生理小种确定...........................................................................................................................................2 7种群动态监测..................................................................................................................................................2 7.1监测内容...................................................................................................................................................3 7.2监测时间...................................................................................................................................................3 7.3数据分析与汇总.......................................................................................................................................3 7.4监测结果...................................................................................................................................................3 8灭活...................................................................................................................................................................3 9档案管理....................................................................................................................