International ISO Standard ISO9299 First edition Traditional Chinese medicine 2024-06 Curcuma longa rhizome Médecine traditionnelle chinoise Rhizome de Curcuma longa Reference number ISO 9299:2024(en) @ISO2024 IS0 9299:2024(en) COPYRIGHT PROTECTED DOCUMENT IS02024 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or Iso's memberbody inthe country ofthe requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+41227490111 Email: [email protected] Website: www.iso.org Published in Switzerland @ IS0 2024 - All rights reserved ii IS0 9299:2024(en) Contents Page Foreword .iv Introduction .V 1 Scope. 1 2 Normative references .1 3 Terms and definitions. .1 .2 4 Descriptions .4 5 Quality and safety requirements and recommendations 5.1 General characteristics 5.2 Macroscopic features 4 5.3 ..4 Microscopic feature. 5.4 Thin-layer chromatography (TLC) features 4 5.5 Marker compounds. .4 5.6 Essential oil .4 5.7 Ethanol-soluble extractive 4 5 5.8 Total ash 5.9 Acid-insoluble ash. 5 5.10 Moisture or loss on drying 5 5.11 Foreign matter 5 5.12 .5 Heavy metals. Pesticide residues 5.13 5 5.14 Sulfur dioxide. 5 5.15 Aflatoxins .5 Sampling 5 6 7 Test methods .5 .5 7.1 Identificationofmacroscopicfeatures 7.2 Identificationofmicroscopicfeatures .5 7.3 .5 Identificationofthin-layerchromatogram 7.4 Determination of marker compounds 6 7.5 Determination of essential oil. .6 7.6 Determination of ethanol-soluble extractive 6 7.7 Determination of total ash 6 7.8 Determination of acid-insoluble ash 7.9 Determination of moisture. .6 7.10 Determination of foreign matter. .6 7.11 Determination of heavy metals .6 Determination of pesticide residues 7.12 ..6 7.13 Determination of sulfur dioxide .6 7.14 Determination of aflatoxin. ..6 8 Test report .6 Packaging, storage and transportation 9 10 Marking and labelling Annex A (informative) Identification of thin-layer chromatogram ..8 .10 AnnexB(informative)Determinationoftotalcontentofcurcuminoids Annex C (informative) Reference of national and regional requirements .12 Annex D (informative) Examples for traditional grading information for Curcuma longa rhizome.... 14 Bibliography .15 @ IS0 2024 - All rights reserved iii