论文标题
广场多光谱光学显微镜的空间均匀照明源
A spatially uniform illumination source for widefield multi-spectral optical microscopy
论文作者
论文摘要
照明均匀性是广场生物成像应用中激发和数据提取质量的关键参数。但是，典型的成像系统由于非理想的光学元件而遭受空间和光谱不均匀性，因此需要复杂的溶液进行照明校正。我们提出有效的均匀颜色集成装置（Euclid），这是一种简单且具有成本效益的照明来源，用于均匀校正。 Euclid采用了弥漫性，可调的空心腔，可从离散的光源中均匀混合光，并修改源场分布，以补偿成像系统中光学成分引入的空间非均匀性。在这项研究中，我们表征了提出的设计的光耦合效率，并将均匀性性能与常规方法进行比较。欧几里得在尼尔森尼和koehler对齐中表现出显着的照明改进，在广泛的视野中，最大空间偏差约为1％。
Illumination uniformity is a critical parameter for excitation and data extraction quality in widefield biological imaging applications. However, typical imaging systems suffer from spatial and spectral non-uniformity due to non-ideal optical elements, thus require complex solutions for illumination corrections. We present Effective Uniform Color-Light Integration Device (EUCLID), a simple and cost-effective illumination source for uniformity corrections. EUCLID employs a diffuse-reflective, adjustable hollow cavity that allows for uniform mixing of light from discrete light sources and modifies the source field distribution to compensate for spatial non-uniformity introduced by optical components in the imaging system. In this study, we characterize the light coupling efficiency of the proposed design and compare the uniformity performance with the conventional method. EUCLID demonstrates a remarkable illumination improvement for multi-spectral imaging in both Nelsonian and Koehler alignment with a maximum spatial deviation of ~1% across a wide field-of-view.
