论文标题
紧凑型同时无标签的无标签自动荧光多谐波（SLAM）显微镜，用于用户友好的光损伤监测成像
Compact simultaneous label-free autofluorescence multi-harmonic (SLAM) microscopy for user-friendly photodamage-monitored imaging
论文作者
论文摘要
无标签的非线性光学显微镜已成为生物医学研究的强大工具。但是，可能的发生损害风险阻碍了进一步的临床应用。为了减少这些不良反应，我们构建了一个新的无标签自动荧光自动荧光多峰（SLAM）显微镜的平台，该平台具有5通道多模式成像，内联光损伤监测和脉冲重复速率调整。通过使用双重光子晶体纤维进行频谱宽扩大（而不是超脑部生成）和棱镜压缩机进行脉冲预磨，该系统允许用户独立地调整脉冲宽度，重复速率和能量，这有助于优化对NO/最小光dodapAmage的幻想条件。此外，它以每个图像像素的一个激发脉冲以一个激发脉冲演示了无标签的多通道成像，从而为通过更快的光学扫描仪改善成像速度铺平了道路。
Label-free nonlinear optical microscopy has become a powerful tool for biomedical research. However, the possible photodamage risk hinder further clinical applications. To reduce these adverse effects, we constructed a new platform of simultaneous label-free autofluorescence multiharmonic (SLAM) microscopy, featuring 5-channel multimodal imaging, inline photodamage monitoring, and pulse repetition-rate tuning. By the use of a birefringent photonic crystal fiber for spectral broadening (rather than supercontinuum generation) and a prism compressor for pulse pre-chirping, this system allows users to independently adjust pulse width, repetition rate, and energy, which is useful for optimizing imaging condition towards no/minimal photodamage. Also, it demonstrates label-free multichannel imaging at one excitation pulse per image pixel and thus paves the way for improving the imaging speed by a faster optical scanner.
