论文标题
分子尺度的底物各向异性和拥挤驱动细胞单层的远程列表
Molecular-scale substrate anisotropy and crowding drive long-range nematic order of cell monolayers
论文作者
论文摘要
细胞对外部刺激的重组能力在从形态发生到组织工程的领域很重要。伸长的细胞可以由于空间效应而共对准，从而形成了局部秩序的状态。我们表明，分子尺度的底物各向异性可以指导细胞组织，从而导致组织尺度上的列表出现。为了定量检查疾病阶的过渡，我们开发了一个高通量成像平台，以分析几天内数千个细胞的速度和定向相关性。通过沿基材列轴增强的细胞分裂以及相关的扩展应力来重组细胞的肌动蛋白网络，从而促进了全局，看似长的顺序。我们的工作连接到一类称为主动干词的系统，为弱相互作用的细胞集体中细胞重塑和组织的动态提供了新的理解。这可以使数据驱动的细胞 - 细胞相互作用发现，并指出组织工程的策略。
The ability of cells to reorganize in response to external stimuli is important in areas ranging from morphogenesis to tissue engineering. Elongated cells can co-align due to steric effects, forming states with local order. We show that molecular-scale substrate anisotropy can direct cell organization, resulting in the emergence of nematic order on tissue scales. To quantitatively examine the disorder-order transition, we developed a high-throughput imaging platform to analyze velocity and orientational correlations for several thousand cells over days. The establishment of global, seemingly long-ranged order is facilitated by enhanced cell division along the substrate's nematic axis, and associated extensile stresses that restructure the cells' actomyosin networks. Our work, which connects to a class of systems known as active dry nematics, provides a new understanding of the dynamics of cellular remodeling and organization in weakly interacting cell collectives. This enables data-driven discovery of cell-cell interactions and points to strategies for tissue engineering.
