论文标题
通过大鼠方法直接重建寿命图
Nanosecond compressive fluorescence lifetime microscopy imaging via the RATS method with a direct reconstruction of lifetime maps
论文作者
论文摘要
事实证明，随机时间信号（大鼠）方法是测量光致发光（PL）动力学和荧光寿命成像（FLIM）的有用且通用的方法。在这里，我们介绍了该方法的两个基本发展步骤。首先，我们证明，通过在大鼠中使用随机的数字激光调制，可以在纳秒单位中以时间分辨率实施PL动力学的测量。其次，我们提出了一种基于单像素摄像机实验的FLIM测量值的替代方法。与标准评估相反，标准评估需要对每个时间点的PL地图进行冗长的迭代重建，在这里我们使用有限的预定PL寿命集并计算与每个生命周期相对应的振幅图。替代方法可显着节省后处理时间，此外，在存在噪声的系统中，它在FLIM频谱图的准确性方面显示出更好的稳定性。除了确认扩展功能的模拟外，我们还将新的进步实施到显微镜光学设置中，以绘制微米尺度上的PL动力学。还通过绘制LUAG：CE晶体表面对所提出的原理进行了实验验证。
RAndom Temporal Signals (RATS) method has proven to be a useful and versatile method for measuring photoluminescence (PL) dynamics and fluorescence lifetime imaging (FLIM). Here, we present two fundamental development steps in the method. First, we demonstrate that by using random digital laser modulation in RATS, it is possible to implement the measurement of PL dynamics with temporal resolution in units of nanoseconds. Secondly, we propose an alternative approach to evaluating the FLIM measurements based on a single-pixel camera experiment. In contrast to the standard evaluation, which requires a lengthy iterative reconstruction of PL maps for each timepoint, here we use a limited set of predetermined PL lifetimes and calculate the amplitude maps corresponding to each lifetime. The alternative approach significantly saves post-processing time and, in addition, in a system with noise present, it shows better stability in terms of the accuracy of the FLIM spectrogram. Besides simulations that confirmed the functionality of the extension, we implemented the new advancements into a microscope optical setup for mapping PL dynamics on the micrometer scale. The presented principles were also verified experimentally by mapping a LuAG:Ce crystal surface.
