论文标题
用纳米孔检测光学单分子检测的钯零模式波导
Palladium Zero-Mode Waveguides for Optical Single Molecule Detection with Nanopores
论文作者
论文摘要
金属膜中的孔会阻止任何传输光，如果波长比孔直径大得多，则建立了所谓的零模式波导（ZMW）之类的纳米孔。另一方面，分子仍然可以通过这些孔通过。我们使用它来检测单个荧光团标记的分子，因为它们穿过ZMW，从而从黑暗区域到发光的一侧穿过，它们会在其上散发出荧光光。这对背景抑制和防止过早漂白都是有益的。我们使用钯作为ZMWS的新型金属膜材料，与常规使用的金属相比，这是有利的。我们证明，可以同时检测不同颜色的单个自由荧光团的易位。在单分子水平上也可以检测到标记的DNA和蛋白质生物分子，并具有高信噪比和高带宽，这为各种单分子生物物理学研究打开了门。
Holes in metal films block any transmitting light if the wavelength is much larger than the hole diameter, establishing such nanopores as so-called Zero Mode Waveguides (ZMWs). Molecules on the other hand, can still passage through these holes. We use this to detect individual fluorophore-labelled molecules as they travel through a ZMW and thereby traverse from the dark region to the illuminated side, upon which they emit fluorescent light. This is beneficial both for background suppression and to prevent premature bleaching. We use palladium as a novel metal-film material for ZMWs, which is advantageous compared to conventionally used metals. We demonstrate that it is possible to simultaneously detect translocations of individual free fluorophores of different colors. Labeled DNA and protein biomolecules can be detected as well at the single-molecule level with a high signal-to-noise ratio and at high bandwidth, which opens the door to a variety of single-molecule biophysics studies.
