论文标题

通过内在的畸变校准精确定位显微镜

Accurate localization microscopy by intrinsic aberration calibration

论文作者

Copeland, Craig R., McGray, Craig D., Ilic, B. Robert, Geist, Jon, Stavis, Samuel M.

论文摘要

定位显微镜的标准范例涉及通过工程信息到发射极图像的延伸到三个维度,以及因光学畸变的现场依赖性而导致的误差的近似。我们颠倒了这个标准范式,引入了通过对普通显微镜的全面校准充分利用内在畸变的潜在信息的概念,从而使整个超消极和深领域的三个维度可以准确地定位单个发射器。为了完成从成像底物到微系统技术的显微镜体的空间信息提取,我们引入了一个协同的概念,即在三个维度上多个发射器位置的刚性转化,提高精度,测试精度和六个自由度的测量测量值。我们的研究阐明了定位显微镜中畸变效应的挑战,将挑战重新定义为准确,准确和完整的定位的机会,并阐明了复杂的微电机械系统的性能和可靠性。

A standard paradigm of localization microscopy involves extension from two to three dimensions by engineering information into emitter images, and approximation of errors resulting from the field dependence of optical aberrations. We invert this standard paradigm, introducing the concept of fully exploiting the latent information of intrinsic aberrations by comprehensive calibration of an ordinary microscope, enabling accurate localization of single emitters in three dimensions throughout an ultrawide and deep field. To complete the extraction of spatial information from microscale bodies ranging from imaging substrates to microsystem technologies, we introduce a synergistic concept of the rigid transformation of the positions of multiple emitters in three dimensions, improving precision, testing accuracy, and yielding measurements in six degrees of freedom. Our study illuminates the challenge of aberration effects in localization microscopy, redefines the challenge as an opportunity for accurate, precise, and complete localization, and elucidates the performance and reliability of a complex microelectromechanical system.

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