论文标题
使用结构化照明眼镜检查的体内视网膜成像中的超分辨率
Super-resolution in vivo retinal imaging using structured illumination ophthalmoscopy
论文作者
论文摘要
结构化照明显微镜（SIM）是最通用的超分辨率技术之一。然而，到目前为止，其用于实时成像的应用主要仅限于荧光和固定标本。在这里，我们介绍了SIM中的进步，以共同应对成像生存样品的所有挑战，即，在处理样本运动，散射，样品诱导的光学畸变和低信噪比的同时，在未呈现的宽场上获得超分辨率。通过使用自适应光学元件来补偿针对运动和厚的组织量身定制的重建算法，我们成功地将SIM应用于体内视网膜成像中，并展示了结构化照明眼镜检查，以在人类视网膜中进行高对比度超分辨率。
Structured illumination microscopy (SIM) is one of the most versatile super-resolution techniques. Yet, its application to live imaging has been so far mainly limited to fluorescent and stationary specimens. Here, we present advancements in SIM to jointly tackle all the challenges of imaging living samples, i.e., obtaining super-resolution over an undistorted wide-field while dealing with sample motion, scattering, sample-induced optical aberrations and low signal-to-noise ratio. By using adaptive optics to compensate for optical aberrations and a reconstruction algorithm tailored for a moving and thick tissue, we successfully applied SIM to in vivo retinal imaging and demonstrated structured illumination ophthalmoscopy for high contrast super-resolution in vivo imaging of the human retina.
