论文标题
水溶液中的单DNA电子自旋共振光谱
Single DNA Electron Spin Resonance Spectroscopy in Aqueous Solutions
论文作者
论文摘要
近乎生理条件下的单个生物分子的磁共振光谱可能会大大提高对生物学功能的理解，但仍然非常具有挑战性。在这里，我们使用钻石中的氮散布中心来检测在环境温度下在水缓冲液溶液中用硝基氧化物旋转标记的个体，束缚DNA双链体的电子自旋谐振光谱。这为单个生物分子及其在类似天然的环境中的分子间相互作用为磁共振研究铺平了道路。
Magnetic resonance spectroscopy of single biomolecules under near-physiological conditions may substantially advance understanding of biological function, yet remains very challenging. Here we use nitrogen-vacancy centers in diamonds to detect electron spin resonance spectra of individual, tethered DNA duplexes labeled with a nitroxide spin label in aqueous buffer solutions at ambient temperatures. This paves the way for magnetic resonance studies on single biomolecules and their inter-molecular interactions in a native-like environment.
