论文标题
单个细胞中蛋白质的纳米级磁共振成像
Nanoscale magnetic resonance imaging of proteins in a single cell
论文作者
论文摘要
磁共振成像（MRI）是一种非侵入性且无标签的技术，广泛用于医学诊断和生命科学研究，其成功从继续在增强对比度和分辨率方面的努力中受益匪浅。在这里，我们报告了使用原子大小量子传感器在单个细胞中的纳米级MRI。在钻石中的氮散布中心的情况下，细胞内蛋白铁蛋白的空间分辨率约为10纳米，并通过相关MRI和电子显微镜共定位了含铁蛋白的细胞器。与当前最新的常规MRI中的当前微米分辨率相比，我们的方法代表了100倍的增强，并为细胞内蛋白的MRI铺平了道路。
Magnetic resonance imaging (MRI) is a non-invasive and label-free technique widely used in medical diagnosis and life science research, and its success has benefited greatly from continuing efforts on enhancing contrast and resolution. Here we reported nanoscale MRI in a single cell using an atomic-size quantum sensor. With nitrogen-vacancy center in diamond, the intracellular protein ferritin has been imaged with a spatial resolution of ~ 10 nanometers, and ferritin-containing organelles were co-localized by correlative MRI and electron microscopy. Comparing to the current micrometer resolution in current state-of-art conventional MRI, our approach represents a 100-fold enhancement, and paves the way for MRI of intracellular proteins.
